Advanced School on Protein Structure and Dynamics: from Spectroscopy to Mass Spectrometry

Parma, February 22-24th, 2017

University of Parma, Interdepartmental Center for Measurements, Department of Food and Drug.

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Dear Colleagues,

we are pleased to announce the 2017 edition of the Italian Association for Crystallography (AIC) International Crystallography School (AICS2017). This year’s school title will be “Bridging the gap between cryo-EM and crystallography”. The school will be held in Pavia, Italy, from 3 to 6 September 2017.

AICS2017 “Bridging the gap between cryo-EM and crystallography” will offer the opportunity to young researchers in biochemistry or structural biology at the PhD/postdoctoral level to approach the fundamental questions arising from the cryo-EM experiment setup. Central topics will include evaluation of sample suitability for analysis and its preparation, choice of appropriate instrumentation for single particle, tomography or electron diffraction approaches, data processing and interpretation of results. A showcase of recent results will contour the tutorial sessions.

Confirmed speakers and tutors will include:
. Wayne Hendrickson (Columbia, US)
. Werner Kuhlbrandt (Max Planck Frankfurt, Germany)
. Martin Walsh (Diamond, UK)
. Fabrizio Martino (CIB Madrid, Spain)
. Tillmann Pape (Imperial College London, UK)
. Marta Carroni (Karolinska, SWE)
. Arjen J. Jakobi (EMBL Heidelberg, Germany)
. B. Tom Burnley (CCP-EM, Diamond, UK)
+ Presentations from Company Specialists about Cryo-EM instrumentations

Registration and practical information can be found at:

http://www.cristallografia.org/aicschool2017

We are making every effort to keep registration fees as low as possible and we offer a very convenient package including school attendance, 4-nights accommodation in historical University Colleges in Pavia (check-in: Sunday, 3 September; check-out: 7 September), lunches and coffee breaks from 4 to 6 September, welcome party on September 3rd, social dinner on September 5th, and a booklet containing copies of the slides used during the lectures for 400 EUR. A number of student bursaries will be available to cover the registration fee in full or partially. The deadline for bursary application is 15 May 2017.

AIC associates will get 50 EUR discount on the registration fee.

Deadline for registration: 15 June 2017.

We look forward to welcoming you in Pavia.

The scientific organizing committee:
Federico Forneris (University of Pavia), Andrea Mattevi (University of Pavia), Filippo Mancia (Columbia University), Martino Bolognesi (University of Milan), Mauro Gemmi (Italian Institute of Technology), Beatrice Vallone (Sapienza University of Rome)

Programme (pdf)

February 22, Course Introduction:

9.30-10.00 Proteins and Biologics –
Silvia Catinella & Andrea Mozzarelli
10.00-11.00 Circular dichroism, proteins and applications –
Luca Ronda
11.00 -11.30 Coffee break
11.30-12.30 Fluorescence, Förster energy transfer, protein conformations and protein-protein interactions –
Stefano Bettati
12.30-14.00 Lunch
14.00-17.30 Practical activities on CD and fluorescence spectra collection, secondary structure determination, protein folding and unfolding
17.30-18.30 Student reading of CD and fluorescence study cases
19.30-21.00 Dinner
21.00-23.00 Selected student presentations of CD and fluorescence study cases

February 23, Chiesi Farmaceutici

9.00-11.00 NMR for protein structure and dynamics –
Serena Faggiano
11.00-11.30 Coffee break
11.30-13.30 Practical activities of NMR experiments
13.30-14.30 Lunch
14.30-15.30 Overview of biological mass spectrometry –
Barbara Pioselli
15.30-16.30 Sample preparation for mass spectrometry –
Laura Tigli & Brenda Rosa
16.30-17.00 Coffee break
17.00-18.30 Student reading of NMR and MS study cases
19.30-21.00 Dinner
21.00-23.00 Selected student presentations of NMR and MS
study cases

February 24, Chiesi Farmaceutici

9.30-10.30 Application of mass spectrometry to determine primary structure of protein: amino acid sequence and post-translational modifications –
Sega Ndiaye (supported by Thermo)
10.30-11.00 Coffee break
11.00-12.00 Mass spectrometry-based tools to analyze protein conformations and dynamics –
Rasmus Uffe Jacobsen (supported by Waters)
12.00-13.00 Application of mass spectrometry for quantification of peptides and proteins –
Samuele Scurati (supported by ABSciex)
13.00-14.00 Lunch
14.00-17.30 MS Practical activities:
– determination of post-translational modifications
– HDX-MS experiments to study protein conformation
– peptide quantitation by mass spectrometry
17.30 Course Conclusions

proteine_school01-gruppo-proteine-small
protein_school_imass

Registration information

The number of participants is limited to 12.

Registration fee: 100 euro (Payment can be made by Credit card or Bank transfer)

Partecipants will be requested to send a short CV including PhD project title

A few fellowships are available for Young SIB Members to cover registration and expenses. Send request to andrea.mozzarelli@unipr.it with CV and motivation letter.

Deadline for registration: January 31, 2017

NB: please, since the number of participants is limited to 12,  wait for acceptance of your registration before proceeding with the payment of the participation fee.

Organizing Committe

Andrea Mozzarelli (UniPR), Stefano Bettati (UniPR), Stefano Bruno (UniPR), Barbara Campanini (UniPR), Luca Ronda (UniPR), Gianluca Paredi (UniPR), Samanta Raboni (UniPR), Serena Faggiano (UniPR), Gianluca Molla (Università Insubria – Varese), Silvia Catinella (Chiesi), Barbara Pioselli (Chiesi), Laura Tigli (Chiesi)

With the support of Chiesi Farmaceutici